Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity
Christopher J. Bergin, Yannick D. Benoit
Abstract
Organoids can enable the study of solid tumors initiated from a single cancer stem cell (CSC) ex vivo. We describe a serial tumor organoid plating protocol using primary colorectal cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic interventions on CSC functions. We detail the isolation of primary colorectal CSCs, organoid embedding, serial passaging, and CSC-related analytical techniques. For complete details on the use and execution of this protocol, please refer to Masibag et al. (2021) and Bergin et al. (2021).
Topics & Concepts
OrganoidColorectal cancerEx vivoCancer stem cellStem cellCancer researchCancerBiologyMedicineIn vivoInternal medicineCell biologyGeneticsCancer Cells and MetastasisCancer Genomics and DiagnosticsChemical Reactions and Isotopes