Measuring local-directional resolution and local anisotropy in cryo-EM maps
José Luis Vilas, Hemant D. Tagare, Javier Vargas, J.M. Carazo, Carlos Óscar S. Sorzano
Abstract
The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation.
Topics & Concepts
Resolution (logic)Projection (relational algebra)Computer sciencePixelImage resolutionMap projectionArtificial intelligenceAlgorithmComputer visionAdvanced Electron Microscopy Techniques and ApplicationsElectron and X-Ray Spectroscopy TechniquesAdvanced X-ray Imaging Techniques