Development of a Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Assay for the Inhibition of Keap1–Nrf2 Protein–Protein Interaction
Sumi Lee, Dhulfiqar Ali Abed, Lesa J. Beamer, Longqin Hu
Topics & Concepts
Förster resonance energy transferKEAP1ChemistrySmall moleculeGlutathioneBiochemistryTranscription factorIn vivoBiophysicsFluorescenceFluorescein isothiocyanateProtein–protein interactionEnzymeBiologyGeneBiotechnologyPhysicsQuantum mechanicsGenomics, phytochemicals, and oxidative stressGlutathione Transferases and PolymorphismsCircadian rhythm and melatonin