Mild membrane depolarization in neurons induces immediate early gene transcription and acutely subdues responses to a successive stimulus
Kira D. A. Rienecker, Robert G. Poston, Joshua S. Segales, Isabelle W. Finholm, Morgan H. Sono, Sorina J. Munteanu, Mina Ghaninejad-Esfahani, Ayna Rejepova, Susana Tejeda-Garibay, Kevin Wickman, Ezequiel Marrón Fernández de Velasco, Stanley A. Thayer, Ramendra N. Saha
Abstract
)-induces a wide array of rapid IEGs and transiently depresses transcriptional and signaling responses to a successive stimulus. This latter effect was independent of de novo transcription, translation, and signaling via calcineurin or mitogen-activated protein kinase. Furthermore, as measured by multiple electrode arrays and calcium imaging, mild depolarization acutely subdues subsequent spontaneous and bicuculline-evoked activity via calcium- and N-methyl-d-aspartate receptor-dependent mechanisms. Collectively, this work suggests that a recent history of graded potential changes acutely depress neuronal intrinsic properties and subsequent responses. Such effects may have several potential downstream implications, including reducing signal-to-noise ratio during synaptic plasticity processes.