Laser Enhanced Combinatorial Chemo-photothermal Therapy of Green Synthesis Gold Nanoparticles Loaded with 6Mercaptopurine on Breast Cancer Model
Amna H. Faid, Samia A. Shouman, Nadia A. Thabet, Yehia Badr, Mahmoud A. Sliem
Abstract
Abstract Purpose We tend to evaluated a method for loading 6Mercaptopurine (6MP) on green synthesized hybrid chitosan gold nanoparticles (AuNPs) forming 6MP-AuNPs nanocomposite for the first time in combinatorial chemo-photothermal therapy. Methods The AuNPs were synthesized using chitosan as a reducing and capping agent. Different concentrations of 6MP were mixed AuNPs. Cells were incubated with 6MP and 6MP loaded AuNPs for 48 h and then exposed to laser. Results AuNPs and 6MP-AuNPs nanocomposite have small sizes of 18 ± 4 and 25 ± 5 nm and exhibit high stability with Zeta potential of 55.9 ± 6.3 and 57 ± 4 mV. 6MP-AuNPs nanocomposite irradiated with Diode Pumped Solid State (DPSS) laser showed a maximum inhibition in cell viability reaching 63% at 1.25 µM. Conclusions A hybrid chitosan gold nanoparticle is a powerful anti-cancer drug carrier as well as photothermal agent in combinatorial chemo-photothermal therapy.