Novel Siwa Propolis and Colistin-Integrated Chitosan Nanoparticles: Elaboration; <i>In Vitro</i> and <i>In Vivo</i> Appraisal
Yosra S.R. Elnaggar, Bassma H. Elwakil, Salma S. Elshewemi, Moustafa Y. El‐Naggar, Adnan A. Bekhit, Zakia A. Olama
Abstract
Aim: The present study aimed to formulate novel cremophore-decorated chitosan nanoparticles of colistin, integrated with Siwa propolis extract, to solve bacterial resistance to colistin. Materials & methods: The novel nanoformula was prepared using an incorporation method. Physicochemical assessment and in vivo studies of the selected nanoformulations were performed. Results: The nanoformulation exhibited a nanosize of 48.3 nm, high ζ potential (43.6 mV), high entrapment efficiency (75%) and complete bacterial growth eradication within 2 h (minimum inhibitory concentration = 6.25 μg/ml). Histological examination showed that incorporation of colistin into the nanoformulation could successfully prevent its nephrotoxicity. Conclusion: Tailoring of proper nanocarrier could successfully revert bacteria from being colistin-resistant to colistin-sensitive. The developed nanoformulation can be considered as a potential antibacterial agent in pneumonia treatment.