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Integration of mRNA and miRNA profiling reveals the heterosis of three hybrid combinations of <i>Capsicum annuum</i> varieties

Sha Yang, Zhuqing Zhang, Wenchao Chen, Xuefeng Li, Shudong Zhou, Chengliang Liang, Xin Li, Bozhi Yang, Xuexiao Zou, Feng Liu, Lijun Ou, Yanqing Ma

2021GM crops & food20 citationsDOIOpen Access PDF

Abstract

from this pathway play a key role in plant development. From the miRNA-seq, the number of clean reads was ranging from 12,132,221 to 25,632,680. A total of 220 miRNAs were predicted in this study, and all of them were identified as novel miRNA. The top three candidate KEGG pathways of miRNA were ribosome signaling pathway (13 miRNAs), spliceosome pathway (13 miRNAs), and plant hormone signal transduction pathways (10 miRNAs). With the mRNA and miRNA integrated analysis, we found some key genes were regulated by some miRNAs. Among them, the scarecrow-like 6 protein can be up or down regulated by mir8, mir120, mir184, mir_214, mir125, and mir130. The function of Della protein was regulated by mir24, mir74, mir94, mir139, and mir190. This study contributes to understanding how heterosis regulates the traits, such as crop production, fruit weight, and fruit length.

Topics & Concepts

BiologymicroRNAHeterosisGeneKEGGGeneticsSignal transductionPlant hormoneComputational biologyGene expressionBotanyTranscriptomeHybridPlant Molecular Biology ResearchGenetic Mapping and Diversity in Plants and AnimalsPlant Stress Responses and Tolerance
Integration of mRNA and miRNA profiling reveals the heterosis of three hybrid combinations of <i>Capsicum annuum</i> varieties | Litcius