Evaluation of a gain-managed nonlinear fiber amplifier for multiphoton microscopy
Pavel Sidorenko, Michael Buttolph, Menansili A. Mejooli, Chi‐Yong Eom, Chris B. Schaffer, Frank W. Wise
Abstract
Two-photon excited fluorescence microscopy is a widely-employed imaging technique that enables the noninvasive study of biological specimens in three dimensions with sub-micrometer resolution. Here, we report an assessment of a gain-managed nonlinear (GMN) fiber amplifier for multiphoton microscopy. This recently-developed source delivers 58-nJ and 33-fs pulses at 31-MHz repetition rate. We show that the GMN amplifier enables high-quality deep-tissue imaging, and furthermore that the broad spectral bandwidth of the GMN amplifier can be exploited for superior spectral resolution when imaging multiple distinct fluorophores.
Topics & Concepts
MicroscopyMaterials scienceOpticsAmplifierOptoelectronicsSpectral imagingOptical amplifierBandwidth (computing)Fluorescence-lifetime imaging microscopyOptical fiberLaserFluorescenceComputer sciencePhysicsTelecommunicationsCMOSAdvanced Fluorescence Microscopy TechniquesOptical Coherence Tomography ApplicationsAdvanced Fiber Laser Technologies