Removal of aflatoxin b1 and t-2 toxin by bacteria isolated from commercially available probiotic dairy foods
Walter Ondiek, Yaling Wang, Lijun Sun, Langhua Zhou, Stephen L. W. On, Haotian Zheng, Gooneratne Ravi
Abstract
This study isolated lactic acid bacteria from commercially available probiotic foods to determine their capacity to remove aflatoxin B 1 (AFB 1 ) and trichothecene-2 (T-2). The removal rates by original live and heat-treated cells of lactic acid bacteria (LAB) were compared to test the effect of heat treatment on efficacy. LAB is capable to remove up to 46% of AFB 1 and up to 45% of T-2 toixn. The toxin removal capability increased as toxin concentration increased despite bacterial cell viability declining. Surprisingly, the denatured LAB removed greater percentages of AFB 1 (up to 62%) and T-2 (up to 52%) than live bacterial cells ( P < 0.05), lending support to the hypothesis that there is higher binding of toxins to the cell membrane of nonviable cells. The research provided practical evidences, which suggest that when ingested into the gut biota, LAB could likely reduce absorption of AFB 1 and T-2 from contaminated foods.