Pump-Probe Time-Resolved Serial Femtosecond Crystallography at X-Ray Free Electron Lasers
Suraj Pandey, Ishwor Poudyal, Tek Narsingh Malla
Abstract
With time-resolved crystallography (TRX), it is possible to follow the reaction dynamics in biological macromolecules by investigating the structure of transient states along the reaction coordinate. X-ray free electron lasers (XFELs) have enabled TRX experiments on previously uncharted femtosecond timescales. Here, we review the recent developments, opportunities, and challenges of pump-probe TRX at XFELs.
Topics & Concepts
FemtosecondLaserFree electron modelX-rayMaterials scienceElectronOpticsChemistryPhysicsQuantum mechanicsPhotosynthetic Processes and MechanismsAdvanced Electron Microscopy Techniques and ApplicationsEnzyme Structure and Function