Subcellular relocalization and nuclear redistribution of the RNA methyltransferases TRMT1 and TRMT1L upon neuronal activation
Nicky Jonkhout, Sonia Cruciani, Helaine Graziele Santos Vieira, Julia Tran, Huanle Liu, Ganqiang Liu, Russell Pickford, Dominik C. Kaczorowski, Glória Regina Franco, Franz Vauti, Noelia Camacho, Seyedeh Sedigheh Abedini, Hossein Najmabadi, Lluı́s Ribas de Pouplana, Daniel Christ, Nicole Schönrock, John S. Mattick, Eva Maria Novoa
Abstract
model that mimics neuronal activation and long term potentiation, we find that both TRMT1 and TRMT1L change their subcellular localization upon neuronal activation. Specifically, we observe a major subcellular relocalization from mitochondria and other cytoplasmic domains (TRMT1) and nucleoli (TRMT1L) to different small punctate compartments in the nucleus, which are as yet uncharacterized. This phenomenon does not occur upon heat shock, suggesting that the relocalization of TRMT1 and TRMT1L is not a general reaction to stress, but rather a specific response to neuronal activation. Our results suggest that subcellular relocalization of RNA modification enzymes may play a role in neuronal plasticity and transmission of information, presumably by addressing new targets.