Balancing Enzyme Encapsulation Efficiency and Stability in Complex Coacervate Core Micelles
Riahna Kembaren, Remco Fokkink, Adrie H. Westphal, Marleen Kamperman, J. Mieke Kleijn, Jan Willem Borst
Abstract
). DLS indeed showed an improved stability of this three-component C3M system against the addition of salt compared to a two-component system. Remarkably, FCS showed that the release of CotA from a three-component C3M system occurred at a lower salt concentration and over a narrower concentration range than the dissociation of C3Ms. In conclusion, although the addition of the homopolymer to the system leads to micelles with a higher salt stability, CotA is excluded from the C3Ms already at lower ionic strengths because the homopolymer acts as a competitor of the enzyme for encapsulation.
Topics & Concepts
CoacervateMicelleDynamic light scatteringChemistryIonic strengthPolyelectrolyteChemical engineeringIodidePolymer chemistryCopolymerChromatographyNanoparticleOrganic chemistryPolymerAqueous solutionEngineeringEnzyme-mediated dye degradationProtein Interaction Studies and Fluorescence AnalysisBiotin and Related Studies