Excellent laccase-like activity of melamine modified Cu-NH2-BDC and selective sensing analyses toward phenols and amines

Abstract

Nanozymes based on metal-organic frameworks (MOFs) have been concentrated on due to their naturally high-disperse metal active sites and the adjustable coordination chemistry. In this work, an N-rich melamine (Mel) was introduced into the Cu-MOF composed of copper(II) nitrate and 2-aminoterephthalic acid (Cu-NH 2 -BDC-Mel) to mimic the laccase, which enzyme-like activities were assessed and applied in sensing analyses toward several phenols and amines. Compared to unmodified Cu-NH 2 -BDC, the resulting Cu-NH 2 -BDC-Mel exhibits enhanced laccase-like activity, superior stability and catalytic kinetics. It is demonstrated that melamine-doping has increased nitrogen content as well as the surface area, as a result, exhibits a lower Michaelis–Menten constant ( K m ) (0.1877 mM) and higher maximum reaction rate ( V max ) (1.7933 × 10 −3 mM·min −1 ) in comparison with that of natural laccase. Based on that, an efficient colorimetric sensing strategy for several phenols and amines was built up with excellent selectivity and anti-interference by using the laccase-like Cu-NH 2 -BDC-Mel, the detection limits are 3.51 µM of adrenaline and 4.41 µM of dopamine. The work broadens the prospect development of bio-colorimetric sensing based on the ligand-modified Cu-MOFs nanozymes catalysis.

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