Diverse bat organoids provide pathophysiological models for zoonotic viruses
Hyunjoon Kim, Hyunjoon Kim, Seo-Young Heo, Young‐Il Kim, Dongbin Park, Monford Paul Abishek N, Suhee Hwang, Yong-Ki Lee, Ho Bin Jang, Jae‐Woo Ahn, Jeongmin Ha, Sujin Park, Ho Young Ji, Se‐Mi Kim, Isaac Choi, Woohyun Kwon, Jaemoo Kim, K.‐S. Kim, Juryeon Gil, Boyeong Jeong, Josea Carmel Lazarte, Rare Rollon, Jeong Ho Choi, Eun-Ha Kim, Seung‐Gyu Jang, Hye Kwon Kim, Hye Kwon Kim, Bo‐Young Jeon, Ghazi Kayali, Richard J. Webby, Bon‐Kyoung Koo, Young Ki Choi, Young Ki Choi
Abstract
Bats are important reservoirs of zoonotic pathogens, but suitable model systems for comprehensively exploring host-pathogen interactions and assessing spillover risks remain limited. To address this gap, we developed a collection of bat organoid models spanning five species and four organ types. This multispecies, multiorgan organoid panel showed species- and tissue-specific replication patterns for several viruses, offering robust pathophysiological models for studying respiratory, renal, and enteric zoonotic viruses. Using this platform, we successfully isolated and characterized bat-borne mammalian orthoreoviruses and paramyxoviruses, demonstrating the utility of these organoid panels for virome surveillance. Furthermore, we successfully tested known antiviral drugs for their efficacy against bat virus isolates.