N6-methyladenosine associated prognostic model in hepatocellular carcinoma
Hanchun Huang, Yi Bai, Xin Lü, Yiyao Xu, Haitao Zhao, Xinting Sang
Abstract
BACKGROUND: N6-methyladenosine (m6A), the most internal mRNA modification, is involved in various cancers. However, the function of m6A in hepatocellular carcinoma (HCC) is still not well explored. Here, we aimed to develop a prognostic model consist of m6A associated genes in HCC. METHODS: The mRNA expression profiles and the corresponding clinical information from the patients with HCC were obtained from The Cancer Genome Atlas (TCGA) database, m6A differentially expressed genes (DEGs) were screened by univariate, Lasso and multivariate Cox regression analyses to develop the prognostic model. The differentially expressed m6A genes in HCC tissues were verified by quantitative reverse transcription PCR (qRT-PCR). RESULTS: ). The time-dependent receiver operating characteristic (ROC) curve analysis confirmed the good performance of this prognostic model (AUC =0.617). After univariate [P<0.001, 1.213 (1.136-1.295)] and multivariate Cox regression analyses [P<0.001, 1.198 (1.115-1.288)] by combined with other clinical factors, this prognostic model was identified as an independent prognostic factor of HCC patients. CONCLUSIONS: The m6A genes were differentially expressed between HCC and normal tissues, and associated with the prognosis of HCC.