Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1
Zhen Li, Bo Hu, Rong Zhou, Xiaojuan Zhang, Ruizhe Wang, Yun Gao, Mingjuan Sun, Binghua Jiao, Lianghua Wang
Abstract
of 64 nM was obtained, while the binding mode of the core sequence and DTX-1 was explored. Based on this aptamer, we developed a biolayer interferometry (BLI) biosensor platform for DTX-1 detection. The aptasensor exhibited a broad detection range from 40 to 600 nM DTX-1 (linear range from 80 to 200 nM), and the low detection limit was 614 pM. Morever, the aptasensor showed good reproducibility and stability, which indicated that this novel aptasensor had broad development prospects for the sensitive and rapid detection of DTX-1.
Topics & Concepts
Selection (genetic algorithm)AptamerBiologyToxicologyGeneticsComputer scienceMachine learningAdvanced biosensing and bioanalysis techniquesbioluminescence and chemiluminescence researchDNA and Nucleic Acid Chemistry