Unraveling the Heterogeneity and Ontogeny of Dendritic Cells Using Single-Cell RNA Sequencing
Binyao Chen, Lei Zhu, Shizhao Yang, Wenru Su
Abstract
Dendritic cells (DCs) play essential roles in innate and adaptive immunity and show high heterogeneity and intricate ontogeny. Advances in high-throughput sequencing technologies, particularly single-cell RNA sequencing (scRNA-seq), have improved the understanding of DC subsets. In this review, we discuss in detail the remarkable perspectives in DC reclassification and ontogeny as revealed by scRNA-seq. Moreover, the heterogeneity and multifunction of DCs during diseases as determined by scRNA-seq are described. Finally, we provide insights into the challenges and future trends in scRNA-seq technologies and DC research.
Topics & Concepts
OntogenyBiologyDendritic cellComputational biologyRNASingle cell sequencingGeneImmunologyGeneticsImmune systemPhenotypeExome sequencingImmunotherapy and Immune ResponsesSingle-cell and spatial transcriptomicsT-cell and B-cell Immunology