Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing
Hsing‐Yi Chung, Ming‐Jr Jian, Chih-Kai Chang, Jung‐Chung Lin, Kuo‐Ming Yeh, Ya‐Sung Yang, Chien‐Wen Chen, Shan‐Shan Hsieh, Shenghui Tang, Cherng‐Lih Perng, Feng‐Yee Chang, Kuo‐Sheng Hung, En–Sung Chen, Mei-Hsiu Yang, Hung‐Sheng Shang
Abstract
of RSV. The assay presented consistent performance in the multicenter study. The multiplex RT-PCR applied on the LabTurbo AIO 48 open platform provided highly sensitive, robust, and accurate results and enabled high-throughput detection of B.1.1.7, influenza A/B, and RSV with short turnaround times. Therefore, this automated molecular diagnostic assay could enable streamlined testing if COVID-19 becomes a seasonal disease.
Topics & Concepts
VirologyMultiplexVirusMedicinePandemicReal-time polymerase chain reactionBiologyCoronavirus disease 2019 (COVID-19)DiseaseInfectious disease (medical specialty)Internal medicineBioinformaticsGeneBiochemistryRespiratory viral infections researchSARS-CoV-2 detection and testingViral gastroenteritis research and epidemiology