TRIBE editing reveals specific mRNA targets of eIF4E-BP in <i>Drosophila</i> and in mammals
Hua Jin, Weijin Xu, Reazur Rahman, Daxiang Na, Allegra Fieldsend, Wei Song, Shaobo Liu, Chong Li, Michael Rosbash
Abstract
and mammals under conditions known to activate 4E-BP. The protein associates with specific mRNAs, and ribosome profiling data show that mTOR inhibition changes the translational efficiency of 4E-BP TRIBE targets more substantially compared to nontargets. In both systems, these targets have specific motifs and are enriched in translation-related pathways, which correlate well with the known activity of 4E-BP and suggest that it modulates the binding specificity of eIF4E and contributes to mTOR translational specificity.
Topics & Concepts
EIF4ERibosome profilingBiologyTranslation (biology)Messenger RNARNA-binding proteinEukaryotic translationTranslational regulationStress granuleCell biologyGeneticsGenePI3K/AKT/mTOR signaling in cancerPolyamine Metabolism and ApplicationsCRISPR and Genetic Engineering