F-actin–rich territories coordinate apoptosome assembly and caspase activation during DNA damage–induced intrinsic apoptosis
Virginia L. King, Kenneth G. Campellone
Abstract
and Apaf-1. The F-actin-rich area also encompasses initiator caspase-9 and clusters of a cleaved form of executioner caspase-3 but restricts accessibility of the caspase inhibitor XIAP. The clustering and potency of caspase-3 activation are positively regulated by the amount of actin polymerized by JMY and WHAMM. These results indicate that JMY-mediated actin reorganization functions in apoptotic signaling by coupling the biogenesis of apoptosomes to the localized processing of caspases.
Topics & Concepts
ApoptosomeBiologyCell biologyActinCytochrome cCaspaseCytoskeletonActin cytoskeletonXIAPLactacystinMitochondrionActin remodelingApoptosisProgrammed cell deathCellBiochemistryProteasomeProteasome inhibitorCell death mechanisms and regulationNuclear Structure and Functioninterferon and immune responses