The Autonomous Glycyl Radical Protein GrcA Restores Activity to Inactive Full-Length Pyruvate Formate-Lyase <i>In Vivo</i>
Michelle Kammel, R. Gary Sawers
Abstract
Many facultative anaerobic microorganisms use glycyl radical enzymes (GREs) to catalyze chemically challenging reactions under anaerobic conditions. Pyruvate formate-lyase (PflB) is a GRE that catalyzes cleavage of the carbon-carbon bond of pyruvate during glucose fermentation.
Topics & Concepts
BiochemistryBiologyFormateCysteineEscherichia coliEnzymeCofactorResidue (chemistry)GlycineAlanineGlycine cleavage systemActive siteEnzyme activatorBeta-galactosidaseAllosteric regulationTransmembrane domainMutantPyruvate dehydrogenase complexEnterobacteriaceaeCleavage (geology)LyaseConserved sequenceMetalloenzymes and iron-sulfur proteinsMicrobial Fuel Cells and BioremediationPorphyrin Metabolism and Disorders