G-quadruplex-guided RNA engineering to modulate CRISPR-based genomic regulation
Xingyu Liu, Shuangyu Cui, Qianqian Qi, Huajun Lei, Yutong Zhang, Weiliang Shen, Fang Fu, Tian Tian, Xiang Zhou
Abstract
It is important to develop small moelcule-based methods to modulate gene editing and expression in human cells. The roles of the G-quadruplex (G4) in biological systems have been widely studied. Here, G4-guided RNA engineering is performed to generate guide RNA with G4-forming units (G4-gRNA). We further demonstrate that chemical targeting of G4-gRNAs holds promise as a general approach for modulating gene editing and expression in human cells. The rich structural diversity of RNAs offers a reservoir of targets for small molecules to bind, thus creating the potential to modulate RNA biology.
Topics & Concepts
BiologyGuide RNACRISPRRNAComputational biologyRNA editingSynthetic biologyG-quadruplexGenome editingGene expressionRegulation of gene expressionGeneNon-coding RNAGeneticsDNACRISPR and Genetic EngineeringRNA and protein synthesis mechanismsAdvanced biosensing and bioanalysis techniques