New insights on CRISPR/Cas9-based therapy for breast Cancer
Hussein Sabit, Shaimaa Abdel-Ghany, Hüseyin Tombuloğlu, Emre Çevik, Amany I. Alqosaibi, Fatma A. Al-Mulhim, Afnan Al-Muhanaa
Abstract
CRISPR/Cas9 has revolutionized genome-editing techniques in various biological fields including human cancer research. Cancer is a multi-step process that encompasses the accumulation of mutations that result in the hallmark of the malignant state. The goal of cancer research is to identify these mutations and correlate them with the underlying tumorigenic process. Using CRISPR/Cas9 tool, specific mutations responsible for cancer initiation and/or progression could be corrected at least in animal models as a first step towards translational applications. In the present article, we review various novel strategies that employed CRISPR/Cas9 to treat breast cancer in both in vitro and in vivo systems.