Improved online LC-MS/MS identification of O-glycosites by EThcD fragmentation, chemoenzymatic reaction, and SPE enrichment
Shuang Yang, Yan Wang, Matthew Mann, Qiong Wang, E Tian, Liping Zhang, John F. Cipollo, Kelly G. Ten Hagen, Lawrence A. Tabak
Topics & Concepts
Electron-transfer dissociationChemistryGlycosylationSerineGlycanThreonineProteasesBiochemistryMass spectrometryChromatographyTandem mass spectrometryGlycoproteinEnzymeGlycosylation and Glycoproteins ResearchAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and Applications