Biocatalytic reductive aminations with NAD(P)H-dependent enzymes: enzyme discovery, engineering and synthetic applications
Bo Yuan, Dameng Yang, Ge Qu, Nicholas J. Turner, Zhoutong Sun
Abstract
similar mechanisms, both involving a carbinolamine intermediate followed by hydride transfer from the co-factor. In addition, both groups catalyze the formation of primary and secondary amines utilizing both organic and inorganic amine donors. In this review, we discuss advances in developing AmDHs and IREDs as biocatalysts and focus on evolutionary history, substrate scope and applications of the enzymes to provide an outlook on emerging industrial biotechnologies of chiral amine production.
Topics & Concepts
Reductive aminationNAD+ kinaseAminationChemistryEnzymeAmine gas treatingBiocatalysisImineCombinatorial chemistryStereochemistryOrganic chemistryBiochemistryCatalysisReaction mechanismEnzyme Catalysis and ImmobilizationChemical Reactions and IsotopesPharmacogenetics and Drug Metabolism