Integrative Biosynthetic Gene Cluster Mining to Optimize a Metabolic Pathway to Efficiently Produce <scp>l</scp>-Homophenylalanine in <i>Escherichia coli</i>
Zhen-Ning Liu, Dengwei Lei, Bin Qiao, Shilin Li, Jianjun Qiao, Guang-Rong Zhao
Abstract
produced 1.41 g/L of l-Hph from glucose in feeding shake-flask fermentation. Our cluster mining strategy enabled optimization of the target metabolic pathway, and it would be promising for production of other valuable products in the postgenomic era.
Topics & Concepts
Escherichia coliGene clusterComputational biologyGeneCluster (spacecraft)Metabolic engineeringSynthetic biologyChemistryBiologyBiochemistryBiotechnologyComputer scienceProgramming languageMicrobial Metabolic Engineering and BioproductionPlant biochemistry and biosynthesisRNA and protein synthesis mechanisms