LeishGEM: genome-wide deletion mutant fitness and protein localisations in Leishmania
Sidonie Aellig, Karen Billington, Jeziel D. Damasceno, Laura Davidson, Ulrich Dobramysl, Ruth Etzensperger, Éden Ramalho Ferreira, Eva Gluenz, Jeremy C. Mottram, Rachel Neish, Raquel Pereira, James Smith, Jack Daniel Sunter, Petr Volf, Richard John Wheeler, Matthew J. Young
Abstract
LeishGEM is a genome-wide functional annotation community resource for Leishmania mexicana, where deletion mutant growth in vitro and in vivo is measured and protein localisation is determined by endogenous tagging and LOPIT-DC (localisation of organelle proteins by isotope tagging with differential centrifugation) spatial proteomics. Data are being made available pre-publication via http://leishgem.org which allows data-driven identification of the mechanisms for Leishmania parasitism.