Identification and Functional Characterization of Fungal Chalcone Synthase and Chalcone Isomerase
Sho Furumura, Taro Ozaki, Akihiro Sugawara, Yohei Morishita, Kento Tsukada, Tatsuya Ikuta, Asuka Inoue, Teigo Asai
Abstract
High Resolution Image Download MS PowerPoint Slide By mining fungal genomic information, a noncanonical iterative type I PKS fused with an N-terminal adenylation–thiolation didomain, which catalyzes the formation of naringenin chalcone, was found. Structural prediction and molecular docking analysis indicated that a C-terminal thioesterase domain was involved in the Claisen-type cyclization. An enzyme responsible for formation of (2 S )-flavanone in the biosynthesis of fungal flavonoids was also identified. Collectively, these findings demonstrate unprecedented fungal biosynthetic machinery leading to plant-like metabolites.
Topics & Concepts
ChalconeFlavanoneChalcone synthaseChalcone isomeraseBiochemistryIsomeraseThioesteraseDocking (animal)EnzymeStereochemistryBiosynthesisChemistryBiologyFlavonoidNursingAntioxidantMedicinePlant biochemistry and biosynthesisPlant Gene Expression AnalysisMicrobial Natural Products and Biosynthesis