Screening of dicyanoisophorone-based probes for highly sensitive detection of viscosity changes in living cells and zebrafish
Fanpeng Kong, Ying Li, Xiao Li, Xiaoxiu Wang, Guanyu Fu, Qiuyue Zhao, Bo Tang
Abstract
simple structural modification of dicyanoisophorone (DCO)-derived dyes. Among them, DCO-5 significantly enhances (180-fold) the response signal in highly viscous aqueous media while showing insensitivity to polarity changes or pH variations, and enables the successful detection of viscosity changes in nystatin-treated HepG2 cells, PC 12 cells and zebrafish.
Topics & Concepts
ZebrafishViscosityIn vivoPolarity (international relations)ChemistrySensitivity (control systems)BiophysicsNanotechnologyMaterials scienceBiochemistryBiologyBiotechnologyGeneCellElectronic engineeringEngineeringComposite materialMolecular Sensors and Ion DetectionLuminescence and Fluorescent MaterialsAnalytical Chemistry and Sensors