Spiral scanning fiber-optic two-photon endomicroscopy with a double-cladding antiresonant fiber
Conghao Wang, huilan liu, Jianrui Ma, Haodong Cui, Yijun Li, Danlei Wu, Yanhui Hu, Dakun Wu, Qiang Fu, Lintang Liang, Fei Yu, Runlong Wu, Aimin Wang, Lishuang Feng
Abstract
Two-photon endomicroscopy is an optical biopsy tool that satisfies clinical requirements for real-time subcellular-resolution imaging to assist pathological biopsy in diagnosis. Herein, we present a two-photon endomicroscopy system based on a piezoelectric ceramic tube scanner. A dual-channel amplitude-modulated sine wave drives the fiber to realize spiral scanning, a double-cladding antiresonant fiber is used for 920-nm femtosecond light-pulse low dispersion transmission, and fluorescence collection occurs with no fiber-tip engineering. The field of view is ∼200 µm, the resolution is 3.1 µm, and the frame rate is 0.7 fps. Pollen grain, GFP-labeled mouse brain section, and human stomach tissue imaging verify the capability of the two-photon endomicroscopy system.