Directed Biosynthesis of Mitragynine Stereoisomers
Carsten Schotte, Yindi Jiang, Dagny Grzech, Thu‐Thuy T. Dang, Larissa C. Laforest, Francisco León, Marco Mottinelli, Satya Swathi Nadakuduti, Christopher R. McCurdy, Sarah E. O’Connor
Abstract
("kratom") is used as a natural remedy for pain and management of opioid dependence. The pharmacological properties of kratom have been linked to a complex mixture of monoterpene indole alkaloids, most notably mitragynine. Here, we report the central biosynthetic steps responsible for the scaffold formation of mitragynine and related corynanthe-type alkaloids. We illuminate the mechanistic basis by which the key stereogenic center of this scaffold is formed. These discoveries were leveraged for the enzymatic production of mitragynine, the C-20 epimer speciogynine, and fluorinated analogues.