Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides
Sarah R. Messenger, Edward M. R. McGuinniety, Luke J. Stevenson, Jeremy G. Owen, Gregory L. Challis, David F. Ackerley, Mark J. Calcott
Topics & Concepts
Nonribosomal peptideMetagenomicsComputational biologyRational designBiologyProtein engineeringPeptideDirected evolutionAdenylylationCombinatorial chemistryBiochemistryChemistryEnzymeBiosynthesisGeneticsGeneMutantMicrobial Natural Products and BiosynthesisGenomics and Phylogenetic StudiesBiochemical and Structural Characterization