FRACTAL: Signal amplification of immunofluorescence <i>via</i> cyclic staining of target molecules
Yehlin Cho, Junyoung Seo, Yeonbo Sim, Jinkyoung Chung, Chan E Park, Chun Gwon Park, Doory Kim, Jae‐Byum Chang
Abstract
In this article, we demonstrate fluorescent signal amplification via cyclic staining of target molecules (FRACTAL), a technique that can amplify the signal intensity of immunofluorescence staining more than nine-fold via simple cyclic staining of secondary antibodies. We also show that FRACTAL is compatible with four-color imaging and expansion microscopy imaging.
Topics & Concepts
StainingSIGNAL (programming language)ImmunofluorescenceMolecular biologyAntibodyBiologyComputer scienceGeneticsProgramming languageAdvanced Fluorescence Microscopy TechniquesAdvanced Biosensing Techniques and ApplicationsGlycosylation and Glycoproteins Research