Suppressor Mutations in LptF Bypass Essentiality of LptC by Forming a Six-Protein Transenvelope Bridge That Efficiently Transports Lipopolysaccharide
Federica A. Falchi, Rebecca J. Taylor, Sebastian J. Rowe, Elisabete C. C. M. Moura, Tiago Baeta, Cédric Laguri, Jean‐Pierre Simorre, Daniel Kahne, Alessandra Polissi, Paola Sperandeo
Abstract
FGC ABC transporter, involved in coordinating energy production and LPS transport. Surprisingly, despite being essential for bacterial growth, LptC can be deleted, provided that a specific residue in the periplasmic domain of LptF is mutated and LptA is overexpressed. Here, we apply biochemical techniques to investigate the suppression mechanism. The data produced in this work disclose an unknown regulatory function of LptF in the transporter that not only expands the knowledge about the Lpt complex but can also be targeted by novel LPS biogenesis inhibitors.
Topics & Concepts
LipopolysaccharideBridge (graph theory)SuppressorBiologyCell biologyGeneticsImmunologyComputational biologyMicrobiologyGeneAnatomyRNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyRNA modifications and cancer