Nanopore Sequencing of the Fungal Intergenic Spacer Sequence as a Potential Rapid Diagnostic Assay
Gretchen A. Morrison, Jianmin Fu, Grace C. Lee, Nathan P. Wiederhold, Connie Cañete‐Gibas, Evelien M. Bunnik, Brian L. Wickes
Abstract
Fungal infections are being caused by a broadening spectrum of fungi, yet in many cases, identification to the species level is required for proper antifungal selection. We investigated the fungal intergenic spacer (IGS) sequence in combination with nanopore sequencing for fungal identification. We sequenced isolates from two Cryptococcus species complexes, C. gattii and C. neoformans , which are the main pathogenic members of this genus, using the Oxford Nanopore Technologies MinION device and Sanger sequencing.
Topics & Concepts
MinionNanopore sequencingBiologyIntergenic regionSanger sequencingCryptococcus neoformansDNA sequencingSequence analysisMicrobiologyGeneticsComputational biologyGenomeGeneFungal Infections and StudiesPlant Pathogens and Fungal DiseasesAntifungal resistance and susceptibility