Self-assembled and perfusable microvasculature-on-chip for modeling leukocyte trafficking
Elisabeth Hirth, Wuji Cao, Marina Peltonen, Edo Kapetanovic, Claudius Dietsche, Sara Svanberg, Maria Filippova, Sai T. Reddy, Petra S. Dittrich
Abstract
We developed and characterized a biologically functional microcirculation system consisting of arterial and venous endothelial cells and pericytes. The model was employed to study leukocyte transendothelial migration in the healthy and inflamed state.
Topics & Concepts
VenuleMicrocirculationVascular permeabilityCell biologyChemistryBasement membraneIntravital microscopyArterioleInflammationChemokineCell adhesion moleculeLeukocyte TraffickingPathologyImmunologyBiologyMedicineInternal medicineAngiogenesis and VEGF in Cancer3D Printing in Biomedical ResearchSingle-cell and spatial transcriptomics