Pushing Boundaries: Introducing Silver-Based Metal–Organic Framework Oxidase-Like Nanozyme over a Wide-Range Temperature
Sameera Sh. Mohammed Ameen, Khalid M. Omer
Abstract
The conventional notion is that enzymes, natural and nanozymes, exhibit peak activity solely within defined psychrophilic or thermophilic conditions. This work challenges the notion by preparing a highly efficient oxidase-mimic of Ag-based metal–organic frameworks (MOFs) (referred to as UoZ-3; UoZ stands for University of Zakho), which work effectively in a wide-range of temperatures. This breakthrough not only widens the working temperature range but also overcomes the instability problem of hydrogen peroxide which limited the use of peroxidase-based applications. UoZ-3 showed lower Michaelis–Menten constants compared to silver nanoparticles and silver ions. Surprisingly, the cold/hot adapted UoZ-3 nanozyme exhibited sensitivity to both hydrogen-atom transfer and single-electron transfer reactions with reducing agents, effectively encompassing all of the antioxidant species that are relevant in physiological contexts. Furthermore, the visual-based platform integrated with a smartphone was established and successfully applied for total antioxidant capacity detection in various temperatures in breast cancer patients. The cold/hot adapted oxidase-mimic nanozyme will open an avenue of nanozyme research and applications.