Coumarin luciferins and mutant luciferases for robust multi-component bioluminescence imaging
Zi Yao, Donald R. Caldwell, Anna Love, Bethany Kolbaba‐Kartchner, Jeremy H. Mills, Martin J. Schnermann, Jennifer A. Prescher
Abstract
direct modification of commercial dyes. The bioluminescent properties of the CouLuc-1 analogs were also characterized, and complementary luciferase enzymes were identified using a two-pronged screening strategy. The optimized enzyme-substrate pairs displayed robust photon outputs and emitted a significant portion of near-infrared light. The CouLuc-1 scaffolds are also structurally distinct from existing probes, enabling rapid multi-component imaging. Collectively, this work provides novel bioluminescent tools along with a blueprint for crafting additional fluorophore-derived probes for multiplexed imaging.
Topics & Concepts
LuciferasesBioluminescenceLuciferaseLuciferinFluorophorePhotoproteinBioluminescence imagingChemistrySubstrate (aquarium)MutantFluorescenceBiophysicsNanotechnologyBiochemistryMaterials scienceBiologyPhysicsOpticsTransfectionGeneEcologybioluminescence and chemiluminescence researchBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniques