Squeeze” enrichment of intact cells (eukaryotic and prokaryotic)from marine sponge tissues prior to routine DNA extraction v1

Abstract

A “Squeeze” method to enrich for intact cells (eukaryotic and prokaryotic) prior to DNA Extraction from marine sponge tissues Most marine demosponge species possess a siliceous skeleton and spicules which can compose a high volume of the total biomass. This skeleton can be a priori removed before standard DNA extraction procedures (with CTAB, proteinase K, and various standard DNA extraction kit protocols). The cell pellets derived from this protocol can be applied in other enrichment protocols prior to standard DNA extraction methods. For example, sponge cells may need to be separated from the large bulk of microbial symbionts by Percoll gradients.

Topics & Concepts