Dynamics of the compartmentalized Streptomyces chromosome during metabolic differentiation
Virginia S. Lioy, Jean-Noël Lorenzi, Soumaya Najah, Thibault Poinsignon, Hervé Leh, Corinne Saulnier, Bertrand Aigle, Sylvie Lautru, Annabelle Thibessard, Olivier Lespinet, Pierre Leblond, Yan Jaszczyszyn, Kévin Gorrichon, Nelle Varoquaux, Ivan Junier, Frédéric Boccard, Jean‐Luc Pernodet, Stéphanie Bury‐Moné
Abstract
Bacteria of the genus Streptomyces are prolific producers of specialized metabolites, including antibiotics. The linear chromosome includes a central region harboring core genes, as well as extremities enriched in specialized metabolite biosynthetic gene clusters. Here, we show that chromosome structure in Streptomyces ambofaciens correlates with genetic compartmentalization during exponential phase. Conserved, large and highly transcribed genes form boundaries that segment the central part of the chromosome into domains, whereas the terminal ends tend to be transcriptionally quiescent compartments with different structural features. The onset of metabolic differentiation is accompanied by a rearrangement of chromosome architecture, from a rather 'open' to a 'closed' conformation, in which highly expressed specialized metabolite biosynthetic genes form new boundaries. Thus, our results indicate that the linear chromosome of S. ambofaciens is partitioned into structurally distinct entities, suggesting a link between chromosome folding, gene expression and genome evolution.