Comparison of MIC Test Strip and Sensititre YeastOne with the CLSI and EUCAST Broth Microdilution Reference Methods for <i>In Vitro</i> Antifungal Susceptibility Testing of Cryptococcus neoformans
Fatima Zohra Delma, Abdullah M. S. Al‐Hatmi, Jochem B. Buil, Hein van der Lee, Marlou Tehupeiory-Kooreman, Sybren de Hoog, Joseph Meletiadis, Paul E. Verweij
Abstract
We compared MIC test strip (MTS) and Sensititre YeastOne (SYO) methods with EUCAST and CLSI methods for amphotericin B, 5-fluocytosine, fluconazole, voriconazole, and isavuconazole against 106 Cryptococcus neoformans isolates. The overall essential agreement between the EUCAST and CLSI methods was >72% and >94% at ±1 and ±2 dilutions, respectively. The essential agreements between SYO and EUCAST/CLSI for amphotericin B, 5-flucytosine, fluconazole, and voriconazole were >89/>93% and between MTS and EUCAST/CLSI were >57/>75%.
Topics & Concepts
Cryptococcus neoformansFlucytosineAmphotericin BVoriconazoleBroth microdilutionMicrobiologyFluconazoleBiologyAntifungalSerial dilutionMedicineMinimum inhibitory concentrationAntibioticsAlternative medicinePathologyFungal Infections and StudiesAntifungal resistance and susceptibilityNail Diseases and Treatments