Physicochemical and biological impact of metal-catalyzed oxidation of IgG1 monoclonal antibodies and antibody-drug conjugates via reactive oxygen species
Zephania Kwong Glover, Aaron T. Wecksler, Baikuntha Aryal, Shrenik Mehta, Melissa A. Pegues, Wayman Chan, Mari Lehtimaki, Allen Luo, Alavattam Sreedhara, V. Ashutosh Rao
Abstract
: DNPH 2,4-dinitrophenylhydrazine; ADC Antibody drug conjugate; ADCC Antibody-dependent cellular cytotoxicity; CDR Complementary determining region; DTT Dithiothreitol; HMWF high molecular weight form; LC-MS Liquid chromatography-mass spectrometry; LMWF low molecular weight forms; MOA Mechanism of action; MCO Metal-catalyzed oxidation; MetO Methionine sulfoxide; mAbs Monoclonal antibodies; MyBPC Myosin binding protein C; ROS Reactive oxygen species; SEC Size exclusion chromatography.
Topics & Concepts
ConjugateMonoclonal antibodyChemistryAntibodyCatalysisReactive oxygen speciesDrugBiochemistryCombinatorial chemistryPharmacologyImmunologyBiologyMathematicsMathematical analysisMonoclonal and Polyclonal Antibodies ResearchCell Adhesion Molecules ResearchHER2/EGFR in Cancer Research