MSD-based assays facilitate a rapid and quantitative serostatus profiling for the presence of anti-AAV antibodies
Janina Haar, Dragica Blazevic, Benjamin Strobel, Sebastian Kreuz, Stefan Michelfelder
Abstract
with high sensitivity and consistency as shown in a screen of sera from 40 healthy human donors. Subsequently, BAb and NAb titers were determined for identification of seronegative animals in a non-human primate (NHP) cohort. Moreover, the MSD-based BAb assay protocol was extended to a panel of 14 different AAV serotypes. In summary, our platform allows a rapid and quantitative assessment of the immunological properties of any natural or engineered AAV variant irrespective of transduction efficiency and enables high-throughput screens.
Topics & Concepts
SerostatusAntibodyBiologyVirologyCapsidTransduction (biophysics)PopulationSerotypeIn vivoImmunoassayImmune systemTiterVirusComputational biologyImmunologyMedicineViral loadGeneticsEnvironmental healthBiochemistryVirus-based gene therapy researchViral Infections and Immunology ResearchHerpesvirus Infections and Treatments