The metal dependence of single-metal mediated phosphodiester bond cleavage: a QM/MM study of a multifaceted human enzyme
Rajwinder Kaur, Mohamed M. Aboelnga, Dylan J. Nikkel, Stacey D. Wetmore
Abstract
significantly changes the metal coordination and active site geometry, and thus completely inhibits catalysis. Our work thereby provides support for the controversial single-metal mediated phosphodiester bond cleavage and clarifies uncertainties regarding the role of the metal and metal identity in this important reaction. This information is key for future medicinal and biotechnological applications including disease diagnosis and treatment, and protein engineering.
Topics & Concepts
Phosphodiester bondChemistryCofactorMetalAP siteQM/MMBond cleavageDeoxyribozymeStereochemistryCleavage (geology)DNAEndonucleaseEnzymeCatalysisBiochemistryOrganic chemistryBiologyRNAGenePaleontologyFracture (geology)Advanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryMetal complexes synthesis and properties