Comprehensive phenotyping of human peripheral blood B lymphocytes in healthy conditions
Rita Carsetti, Sara Terreri, Maria Giulia Conti, Ane Fernandez Salinas, Francesco Corrente, Claudia Capponi, Christian Albano, Eva Piano Mortari
Abstract
The B cell compartment provides innate and adaptive immune defenses against pathogens. Different B cell subsets, reflecting the maturation stages of B cells, have noninterchangeable functions and roles in innate and adaptive immune responses. In this review, we provide an overview of the B cell subsets present in peripheral blood of healthy individuals. A specific gating strategy is also described to clearly and univocally identify B cell subsets based on the their phenotypic traits by flow cytometric analysis.
Topics & Concepts
BiologyImmune systemImmunologyFlow cytometryB cellInnate immune systemPhenotypePeripheral bloodAcquired immune systemCellPeripheralCompartment (ship)Cell biologyMedicineGeneticsGeneAntibodyOceanographyGeologyInternal medicineT-cell and B-cell ImmunologyImmune Cell Function and InteractionImmune Response and Inflammation