Megapixel scanning transmission soft X-ray microscopy imaging coupled with compressive sensing X-ray fluorescence for fast investigation of large biological tissues
George Kourousias, Fulvio Billè, Roberto Borghes, Lorella Pascolo, Alessandra Gianoncelli
Abstract
Soft X-ray microscopy coupled with low energy X-ray fluorescence is a powerful tool for investigating complex biological systems like cells and tissues. Due to certain characteristics of X-ray sources, sample stage motors, and detectors, the examination of large areas at high resolutions is very time consuming, often confining the analysis only to a restricted number of pre-selected representative regions. Here we propose and demonstrate a compressive sensing method that provides an alternative approach for overcoming such limitations and can be applied to different kinds of samples and other microscopy and analytical techniques.
Topics & Concepts
FluorescenceX-rayMicroscopyFluorescence microscopeCompressed sensingX-ray fluorescenceMaterials scienceTransmission electron microscopyBiological specimenOpticsNanotechnologyComputer sciencePhysicsArtificial intelligenceAdvanced MRI Techniques and ApplicationsMedical Imaging Techniques and ApplicationsAdvanced X-ray Imaging Techniques