Molecular characterization of superbugs K. pneumoniae harboring extended-spectrum β-lactamase (ESBL) and carbapenemase resistance genes among hospitalized patients in southwestern Iran, Western Asia
Farhad Moradi, Maryam Akbari, Roghayyeh Vakili‐Ghartavol, M I Ostovari, Nahal Hadi
Abstract
Background: in two hospitals located in Southwest Iran. Methods: isolates that exhibited resistance to at least one of the three indicator cephalosporins or carbapenems were selected for evaluation of ESBL and carbapenemase production. This was done using a combination disk confirmatory test and the modified carbapenem inactivation method (mCIM). Finally, the presence of ESBLs and carbapenemase resistance encoding genes was assessed using PCR and specific primers. Results: resistant genes among clinical isolates. Conclusion: resistant strains should be addressed.
Topics & Concepts
Klebsiella pneumoniaeMicrobiologyAntibiotic resistanceGeneBiologyAntibioticsMedicineGeneticsEscherichia coliAntibiotic Resistance in BacteriaAntibiotic Use and ResistanceBacterial Identification and Susceptibility Testing