Plants and microorganisms both contribute to soil organic matter formation through mineral interactions: Evidence from a subtropical forest succession
Yiren Zhu, Minghui Hu, Dafeng Hui, Guoxiang Niu, Jianling Li, Xianyu Yao, Yuanliu Hu, Xiaolin Huang, Yonghui Li, Deqiang Zhang, Qi Deng
Abstract
• SOC accumulation with succession was accompanied by increasing contributions of MAOC. • Microbial necromass increased from early to middle stage but not at the climax stage. • Contributions of lignin phenols and LF + POC to SOC declined with forest succession. • Both plant and microbial pathways contributed to SOC formation. Understanding the formation and stabilization of soil organic carbon (SOC) is essential for predicting SOC dynamics. Traditionally, it was believed that SOC accumulates primarily through the selective retention of recalcitrant plant lignin components. However, an emerging hypothesis suggests that microbial necromass adsorbed onto mineral-associated soil fractions play a more significant role in promoting SOC formation. In this study, we tested the above hypothesis by investigating SOC content, particulate fraction (LF + POC) vs. mineral-associated fraction (MAOC), along with microbial necromass (amino sugars as biomarker) and plant lignin component (lignin phenols as biomarker) in the topsoil (0–20 cm) and subsoil (20–40 cm) across three successional stages: early coniferous forest, middle mixed forest and climax broadleaved forest in southern China. Results showed that SOC content increased with forest succession, accompanied by increasing contributions of MAOC in both soil layers. Interestingly, the contribution of microbial necromass to SOC increased throughout the succession only in the subsoil, whereas in the topsoil, it increased from the early to the middle stage, then slightly decreased at the climax stage. Additionally, the contributions of lignin phenols or LF + POC to SOC decreased in both soil layers with forest succession. A partial least squares path model further revealed that MAOC played a dominate role in governing SOC accumulation, driven by active mineral content combined with plant-derived dissolved organic matter in the topsoil and microbial necromass in the subsoil. Collectively, our findings suggest that plants and microorganisms contribute to SOC formation through interactions with minerals, unveiling an intricate interactive mechanism of plant–microbe-mineral continuum in SOC stabilization.