Profiling of branched-chain fatty acids <i>via</i> nitroxide radical-directed dissociation integrated on an LC-MS/MS workflow
Ruijun Jian, Xue Zhao, Qiaohong Lin, Yu Xia
Abstract
characteristic 28 Da spacing due to cleavages on either side of the branching point, with enhanced fragmentation observed toward the carbonyl end. This nitroxide-RDD method has been integrated onto reversed-phase liquid chromatography and applied for the profiling of BCFAs from yak milk powder and pooled human plasma samples. Other than the more often encountered iso- and anteiso-BCFAs, we have identified FA n-5 17 : 0 as a minor component from human plasma, which has been rarely reported before.
Topics & Concepts
ChemistryNitroxide mediated radical polymerizationBranching (polymer chemistry)Dissociation (chemistry)Tandem mass spectrometryRadicalAdductMass spectrometryChromatographyFragmentation (computing)Organic chemistryRadical polymerizationPolymerizationOperating systemPolymerComputer scienceMetabolomics and Mass Spectrometry StudiesMass Spectrometry Techniques and ApplicationsFatty Acid Research and Health