The maize single-nucleus transcriptome comprehensively describes signaling networks governing movement and development of grass stomata
Guiling Sun, Mingzhang Xia, Jieping Li, Wen Ma, Qingzeng Li, Jinjin Xie, Shenglong Bai, Shanshan Fang, Ting Sun, Xinlei Feng, Guanghui Guo, Yanli Niu, Jingyi Hou, Wenling Ye, Jianchao Ma, Siyi Guo, Hongliang Wang, Yu Long, Xuebin Zhang, Junli Zhang, Hui Zhou, Baozhu Li, Jiong Liu, Changsong Zou, Hai Wang, Jinling Huang, David W. Galbraith, Chun‐Peng Song
Abstract
The unique morphology of grass stomata enables rapid responses to environmental changes. Deciphering the basis for these responses is critical for improving food security. We have developed a planta platform of single-nucleus RNA-sequencing by combined fluorescence-activated nuclei flow sorting, and used it to identify cell types in mature and developing stomata from 33,098 nuclei of the maize epidermis-enriched tissues. Guard cells (GCs) and subsidiary cells (SCs) displayed differential expression of genes, besides those encoding transporters, involved in the abscisic acid, CO2, Ca2+, starch metabolism, and blue light signaling pathways, implicating coordinated signal integration in speedy stomatal responses, and of genes affecting cell wall plasticity, implying a more sophisticated relationship between GCs and SCs in stomatal development and dumbbell-shaped guard cell formation. The trajectory of stomatal development identified in young tissues, and by comparison to the bulk RNA-seq data of the MUTE defective mutant in stomatal development, confirmed known features, and shed light on key participants in stomatal development. Our study provides a valuable, comprehensive, and fundamental foundation for further insights into grass stomatal function.